doi: 10.1016/0014-4800(65)90015-8, He, D., Chung, M., Chan, E., Alleyne, T., Ha, K. C. H., Miao, M., et al. (1973). Tropoelastin: a versatile, bioactive assembly module - PubMed Biol. doi: 10.1161/circimaging.117.007303, Prhashanna, A., Taylor, P. A., Qin, J., Kiick, K. L., and Jayaraman, A. Codistribution analysis of elastin and related fibrillar proteins in early vertebrate development. doi: 10.1074/jbc.M113.518381, Lee, P., Yeo, G. C., and Weiss, A. S. (2017). Biopolymers 103, 563573. doi: 10.1016/j.jsb.2005.02.005, Martin, S. L., Vrhovski, B., and Weiss, A. S. (1995). To get started with texting, you will need: Your phone number 15, 786801. (1983). Elastases can be in the form of serine- (e.g., Ela-2), cysteine- (e.g., cathepsin I), or matrix metalloproteinases (MMP-2, -7, -9, and -12), and bioactive peptides include the VGVAPG peptide. (2007). Its estimated half-life of 70 years (Shapiro et al., 1991) is due to its extensive cross-linking and high hydrophobicity, which render it resistant to degradation (Vrhovski and Weiss, 1998; Schrader et al., 2018; Hedtke et al., 2019). Trends towards maximal association are apparent when each of these parameters is varied. doi: 10.1128/mcb.26.5.1700-1709.2006, Mecham, R. P. (1991). Acta Histochem. Sci. Biochim. doi: 10.1016/j.matbio.2006.09.011, Miao, M., Reichheld, S. E., Muiznieks, L. D., Huang, Y., and Keeley, F. W. (2013). Chem. doi: 10.1021/acs.biomac.8b01503, Rauscher, S., Baud, S., Miao, M., Keeley, F. W., and Poms, R. (2006). Background: Elastin is an essential component of the dermis, providing skin with elasticity and integrity. Biomacromolecules 18, 599609. Developing elastic tissue. Pepe, A., Flamia, R., Guerra, D., Quaglino, D., Bochicchio, B., Pasquali Ronchetti, I., et al. Proline-poor hydrophobic domains modulate the assembly and material properties of polymeric elastin. This canonical computational structure was determined to be highly similar to the envelope found via SAXS/SANS, where both consist of an extended molecular body accompanied by a protruding foot (Baldock et al., 2011). Allergan acquired Elastagen primarily for the injectable product portfolio (acne scars/stretch marks aesthetics). A., Starcher, B. C., and Urry, D. W. (1974). Even after being able to produce recombinant tropoelastin at scales suited to characterization studies, traditional techniques have not yet produced an experimentally verified atomistic structure (Martin et al., 1995; Tarakanova and Buehler, 2013). doi: 10.1073/pnas.1215779110, Noguchi, A., Firsching, K., Kursar, J. D., and Reddy, R. (1990). Micron 41, 268272. Biophys. Mater. doi: 10.1111/j.1749-6632.1991.tb17013.x, Mecham, R. P., Levy, B. D., Morris, S. L., Madaras, J. G., and Wrenn, D. S. (1985). doi: 10.1371/journal.pone.0046130, Hedtke, T., Schrader, C. U., Heinz, A., Hoehenwarter, W., Brinckmann, J., Groth, T., et al. J. Therm. Soluble matrix protein is a potent modulator of mesenchymal stem cell Matrix Biol. 10:034102. doi: 10.1088/1748-6041/10/3/034102, Hinek, A., Keeley, F. W., and Callahan, J. Allergan to acquire University of Sydney spinoff Elastagen This review focuses on recent developments that have shaped our current knowledge of elastin assembly through understanding the relationship between tropoelastins structure and function. Mater. Self-healing and thermoreversible rubber from supramolecular assembly. US8974803B2 - Injectable biomaterials - Google Patents (2017). Elastin - an overview | ScienceDirect Topics 280, 3052630537. 264, 88878891. doi: 10.1021/bi00835a022, Lockhart-Cairns, M. P., Newandee, H., Thomson, J., Weiss, A. S., Baldock, C., and Tarakanova, A. Biopolymers 107:e23007. Biol. EBP plays two roles in the assembly of elastin. Indeed, cryo-EM was recently reported to have a lower molecular size limit of approximately 50 kDa, however, it has not yet been successfully applied to characterize tropoelastin (Murata and Wolf, 2018). In addition to interacting with tropoelastin, fibulin-4 and -5 are capable of also binding LOX and fibrillin-1 and, thus, have key roles in facilitating elastogenesis (Hinderer et al., 2015). Tropoelastin. 11 Articles, This article is part of the Research Topic, Tropoelastin-Cell Receptor Interactions and Implications for Assembly, https://doi.org/10.3389/fbioe.2021.643110, Creative Commons Attribution License (CC BY). Tropoelastin is characterised by alternating hydrophobic and hydrophilic domains and is highly flexible. doi: 10.1074/jbc.M113.533893, Reiersen, H., and Rees, A. R. (2000). Subtle balance of tropoelastin molecular shape and flexibility regulates dynamics and hierarchical assembly. 26, 125135. Elastogenesis occurs during replacement fibrosis in human heart disease and can be accelerated in vitro via tropoelastin treatment. Elastin: Structure, Function, Skin Care & Supplements - Cleveland Clinic The hydrophobic domains vary in length, with the majority of the shorter (95 residues) domains occurring near the N-terminus, while the longer (up to 55 residues) domains are found within tropoelastins central and C-terminal regions (Indik et al., 1989). Elastic fibers are eventually formed after extensive cross-linking through a process termed maturation (Yeo et al., 2016). (2019b). Calorim. J. Biochem. Dermatol. Isolation and characterization of crosslinked peptides from elastin. doi: 10.1073/pnas.1801205115, Tarakanova, A., Yeo, G. C., Baldock, C., Weiss, A. S., and Buehler, M. J. A Study to Evaluate the Efficacy of ELAPR002f in Females and Males With The process of tropoelastin aggregation is initially reversible, as spherules dissipate if the temperature is lowered (Clarke et al., 2006), however, maintenance of a physiological temperature results in maturation, which is indicated by spherule coalescence and the irreversible formation of fibrillar structures (Cox et al., 1974; Bressan et al., 1983; Mithieux et al., 2005). 275, 2844928454. Res. For more information, visit Elastagens website atwww.elastagen.com. Res. Crucially, these simulations revealed that self-assembly starts with a nucleation event and elongation proceeds via both globular and fibrillar structures (Tarakanova et al., 2019a). The stiffness that arose due to amino acid substitutions partially determined resultant secondary structure which, in turn, impacted assembly (Prhashanna et al., 2019). Proc. Direct observation of structure and dynamics during phase separation of an elastomeric protein. A series of mapping studies have been recently conducted to pinpoint the locations of these cross-links (Schrader et al., 2018; Hedtke et al., 2019; Schmelzer et al., 2019) as their placements are crucial to understanding the resultant molecular orientation of tropoelastin within cross-linked elastin. Human tropoelastin improves rat heart function postmyocardial infarction and could potentially be used to treat heart failure. Attempts to characterize tropoelastins structure have seen a gradual shift from the belief that tropoelastins domains have fixed structures, to understanding that the majority of its domains are capable of transitioning between random coils and transient ordered structures. J. Biol. Front. Structural changes and facilitated association of tropoelastin. J. Biol. Elastagens revolutionary recombinant human tropoelastin, is identical to that present in human tissue and has many clinical applications, including effective breakthrough treatments for acne scars, stretch marks, aesthetic skin repair and wound repair. A., Wachi, H., Davis, E. C., and Mecham, R. P. (2003). Characterization of an unusual tropoelastin with truncated C-terminus in the frog. doi: 10.1002/bip.1981.360200611. CB gratefully acknowledges BBSRC funding (Ref: BB/R008221/1). While tropoelastin is a flexible molecule, a combination of optical and structural analyses has defined key regions of the molecule that directly contribute to the elastomeric properties and control the cell interactions of the protein. Biochemistry 8, 28372845. Indeed, studies examining the consequences of domain insertions and deletions note changes in the intrinsic functionality of tropoelastin, corroborating the hypothesis that domain insertions and deletions result in altered tissue mechanics (Jensen et al., 2000; Kozel et al., 2003; Yeo et al., 2016; Miao et al., 2017). The N-terminal head caps an extended coil region that consists of domains 218. Biotechnol. Forty tropoelastin molecules were subjected to simulations using a MARTINI-based coarse-grained model, integrated with an elastic network model (Tarakanova et al., 2019a). FEBS J. (2012). Chem. Sci. (2016). The amino acid sequence of domain 36 is unique; furthermore, its sequence confers a positively charged C-terminus comprising lysines between positively charged arginines, forming a RKRK sequence (Vrhovski et al., 1997). Trifluoroethanol may form a solvent matrix for assisted hydrophobic interactions between peptide side chains. Sci. Cell Physiol. doi: 10.1016/j.str.2006.09.008, Rauscher, S., and Pomes, R. (2017). Tropoelastin Improves Post-Infarct Cardiac Function doi: 10.1073/pnas.1701877114, Reichheld, S. E., Muiznieks, L. D., Lu, R., Sharpe, S., and Keeley, F. W. (2019). doi: 10.1021/bi034837t, Tamburro, A. M., Guantieri, V., and Gordini, D. D. (1992). Biochimie 86, 173178. Commun. Closer to the base of the molecule, the flexible hinge region between domains 21 and 23 presented with a scissors-like bending, which is now believed to contribute to elasticity and multimeric assembly through enhanced conformational space sampling (Tarakanova et al., 2019b). J. Biol. Hogan Lovells represented Elastagen Pty Ltd. on the deal. Acta Gen. Subj. Natl. An electron microscopic study. 249, 997998. Mol. Fabricating organized elastin in vascular grafts. Trends Biotechnol. Allergan markets a portfolio of leading brands and best-in-class products for the central nervous system, eye care, medical aesthetics and dermatology, gastroenterology, womens health, urology and anti-infectivetherapeutic categories. The elastin receptor complex: a unique matricellular receptor with high anti-tumoral potential. Banded fibers in tropoelastin coacervates at physiological temperatures. Sci. doi: 10.1126/sciadv.1501145, Yeo, G. C., and Weiss, A. S. (2019). 1862, 324334. Am. doi: 10.1007/978-3-319-71530-8_13-1, Wen, Q., Mithieux, S. M., and Weiss, A. S. (2020). Rev. Tropoelastins sequence and domain arrangement. doi: 10.1016/j.matbio.2006.10.002, Dyksterhuis, L. B., and Weiss, A. S. (2010). Soluble matrix protein is a potent modulator of mesenchymal stem cell performance. doi: 10.1038/nrm3904, Bressan, G. M., Castellani, I., Giro, M. G., Volpin, D., Fornieri, C., and Pasquali Ronchetti, I. Stages in tropoelastin coalescence during synthetic elastin hydrogel formation. Elastin is heterogeneously cross-linked. Today Biol. Computational approaches have gained popularity in recent decades as their methodologies have been refined to provide accurate atomistic scale insights into molecular structure and movement (Tarakanova et al., 2018). Powered by Madgex Job Board Software. Tropoelastins flexibility does not allow it to pack into a crystal lattice and its size renders it too complex to assign atomic interactions using nuclear magnetic resonance (NMR) on a global level (Tamburro et al., 2003, 2006; Bochicchio et al., 2004; Pepe et al., 2008). 252, 351365. (2015). Lyophilized Powder. N. Y. Acad. Tropoelastin - PubMed Computational smart polymer design based on elastin protein mutability. Biochemistry 52, 77317741. Proc. Ann. Patient Homepage | Accredo Further down the molecule, the cell-interactive C-terminus was noted to be highly flexible, indicating that high conformational sampling could be conducive to tropoelastins interactions between cell-surface receptors and elastic fiber-associated proteins. Elastin as a random-network elastomer: a mechanical and optical analysis of single elastin fibers. Struct. A., Rongish, B. J., Czirok, A., Zach, J., Little, C. D., Davis, E. C., et al. Characterization of the complete human elastin gene. For example, the highly flexible domains 25 that were noted to generate a twisting motion in N-terminus, were positioned beside domain 6, a relatively immobile region that may assist in stabilizing the subsequent regions. 87, 18281834. Lysinonorleucine. Stages of hierarchical assembly of elastic fibers. (2018). Tropoelastins amino acid sequence predominantly consists of non-polar residues including glycine, valine, alanine, and proline (Debelle and Tamburro, 1999). Sci. doi: 10.1016/j.bbrc.2010.05.013, Elvin, C. M., Carr, A. G., Huson, M. G., Maxwell, J. M., Pearson, R. D., Vuocolo, T., et al. into 200 nm then 12 m spherules, which eventually grow and stabilize into spherules 26 m in diameter (Clarke et al., 2006; Kozel et al., 2006; Tu and Weiss, 2010; Tu et al., 2010). Cell Biol. Cell Biol. 300, 13351359. 21:063001. doi: 10.1088/0965-0393/21/6/063001, Tarakanova, A., Huang, W., Weiss, A. S., Kaplan, D. L., and Buehler, M. J. Thus, computational approaches can be considered as important means to complement wet bench experiments. 34, 8190. II. Further to this, the flexibility of the hydrophobic domains may also play a key role in self-assembly. Eng. Proc. The majority of tropoelastin synthesis occurs during perinatal development (Myers et al., 1983; Noguchi et al., 1990), however, synthesis may be triggered in response to tissue damage (Parks et al., 1992) or during diseases such as atherosclerosis (Phinikaridou et al., 2018). Natl. doi: 10.1021/acs.biomac.0c00546, Bochicchio, B., Floquet, N., Pepe, A., Alix, A. J., and Tamburro, A. M. (2004). Biopolymers 29, 16991706. (1965). Together, these two properties can be tuned and exploited to give rise to an increasing number of novel biomaterials for tissue engineering and regenerative medicine, which have been recently reviewed elsewhere (Wang et al., 2020; Wen et al., 2020). doi: 10.1016/j.matbio.2006.08.258, Clarke, A. W., Arnspang, E. C., Mithieux, S. M., Korkmaz, E., Braet, F., and Weiss, A. S. (2006). The presence of tropoelastin spherules fusing to fibrils has been noted in native tissue, demonstrating marked similarities between in vitro and in vivo coacervation (Haust et al., 1965; Albert, 1972; Kozel et al., 2006). (2005). Chem. doi: 10.1046/j.1432-1327.1999.00891.x, Yamauchi, Y., Tsuruga, E., Nakashima, K., Sawa, Y., and Ishikawa, H. (2010).
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